<html lang="en"> <head> <title>Iron-regulatory proteins secure iron availability in cardiomyocytes to prevent heart failure</title> <meta charset="utf-8"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta http-equiv="X-UA-Compatible" content="ie=edge"> <link href="https://unpkg.com/@stencila/thema@2/dist/themes/stencila/styles.css" rel="stylesheet"> <script src="https://unpkg.com/@stencila/thema@2/dist/themes/stencila/index.js" type="text/javascript"></script> <script src="https://unpkg.com/@stencila/components@<=1/dist/stencila-components/stencila-components.esm.js" type="module"></script> <script src="https://unpkg.com/@stencila/components@<=1/dist/stencila-components/stencila-components.js" type="text/javascript" nomodule=""></script> </head> <body> <main role="main"> <article itemscope="" itemtype="http://schema.org/Article" data-itemscope="root"> <h1 itemprop="headline">Iron-regulatory proteins secure iron availability in cardiomyocytes to prevent heart failure</h1> <meta itemprop="image" content="https://via.placeholder.com/1200x714/dbdbdb/4a4a4a.png?text=Iron-regulatory%20proteins%20secure%20iron%20availability%20in%20cardiomyocytes%20to%20prevent%20heart%20failure"> <ol data-itemprop="authors"> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Saba Haddad"><span data-itemprop="givenNames"><span itemprop="givenName">Saba</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Haddad</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Yong Wang"><span data-itemprop="givenNames"><span itemprop="givenName">Yong</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Wang</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Bruno Galy"><span data-itemprop="givenNames"><span itemprop="givenName">Bruno</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Galy</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Mortimer Korf-Klingebiel"><span data-itemprop="givenNames"><span itemprop="givenName">Mortimer</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Korf-Klingebiel</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Valentin Hirsch"><span data-itemprop="givenNames"><span itemprop="givenName">Valentin</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Hirsch</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Abdul M. Baru"><span data-itemprop="givenNames"><span itemprop="givenName">Abdul</span><span itemprop="givenName">M.</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Baru</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Fatemeh Rostami"><span data-itemprop="givenNames"><span itemprop="givenName">Fatemeh</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Rostami</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Marc R. Reboll"><span data-itemprop="givenNames"><span itemprop="givenName">Marc</span><span itemprop="givenName">R.</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Reboll</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Jörg Heineke"><span data-itemprop="givenNames"><span itemprop="givenName">Jörg</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Heineke</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Ulrich Flögel"><span data-itemprop="givenNames"><span itemprop="givenName">Ulrich</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Flögel</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Stephanie Groos"><span data-itemprop="givenNames"><span itemprop="givenName">Stephanie</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Groos</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="André Renner"><span data-itemprop="givenNames"><span itemprop="givenName">André</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Renner</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Karl Toischer"><span data-itemprop="givenNames"><span itemprop="givenName">Karl</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Toischer</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Fabian Zimmermann"><span data-itemprop="givenNames"><span itemprop="givenName">Fabian</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Zimmermann</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Stefan Engeli"><span data-itemprop="givenNames"><span itemprop="givenName">Stefan</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Engeli</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Jens Jordan"><span data-itemprop="givenNames"><span itemprop="givenName">Jens</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Jordan</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Johann Bauersachs"><span data-itemprop="givenNames"><span itemprop="givenName">Johann</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Bauersachs</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Matthias W. Hentze"><span data-itemprop="givenNames"><span itemprop="givenName">Matthias</span><span itemprop="givenName">W.</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Hentze</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Kai C. Wollert"><span data-itemprop="givenNames"><span itemprop="givenName">Kai</span><span itemprop="givenName">C.</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Wollert</span></span> </li> <li itemscope="" itemtype="http://schema.org/Person" itemprop="author"> <meta itemprop="name" content="Tibor Kempf"><span data-itemprop="givenNames"><span itemprop="givenName">Tibor</span></span><span data-itemprop="familyNames"><span itemprop="familyName">Kempf</span></span> </li> </ol><span itemscope="" itemtype="http://schema.org/Organization" itemprop="publisher"> <meta itemprop="name" content="Unknown"><span itemscope="" itemtype="http://schema.org/ImageObject" itemprop="logo"> <meta itemprop="url" content="https://via.placeholder.com/600x60/dbdbdb/4a4a4a.png?text=Unknown"> </span> </span><time itemprop="datePublished" datetime="2017-02-01">2017-02-01</time> <ul data-itemprop="genre"> <li itemprop="genre">Article</li> </ul> <ul data-itemprop="keywords"> <li itemprop="keywords">Iron deficiency</li> <li itemprop="keywords">Heart failure</li> <li itemprop="keywords">Energy metabolism</li> <li itemprop="keywords">Extracellular flux analysis</li> <li itemprop="keywords">31P-Magnetic resonance spectroscopy</li> </ul> <ul data-itemprop="identifiers"> <li itemscope="" itemtype="http://schema.org/PropertyValue" itemprop="identifier"> <meta itemprop="propertyID" content="https://registry.identifiers.org/registry/doi"> <span itemprop="name">doi</span><span itemprop="value">10.1093/eurheartj/ehw333</span> </li> </ul> <section data-itemprop="description"> <h2 data-itemtype="http://schema.stenci.la/Heading">Abstract</h2> <meta itemprop="description" content="Iron deficiency (ID) is associated with adverse outcomes in heart failure (HF) but the underlying mechanisms are incompletely understood ..."> <p itemscope="" itemtype="http://schema.stenci.la/Paragraph">Iron deficiency (ID) is associated with adverse outcomes in heart failure (HF) but the underlying mechanisms are incompletely understood ...</p> </section> <h2 itemscope="" itemtype="http://schema.stenci.la/Heading" id="results">Results</h2> <h3 itemscope="" itemtype="http://schema.stenci.la/Heading" id="reduced-iron-content-ire-binding-activity-and-transferrin-receptor-expression-in-the-failing-human-heart"> Reduced iron content, IRE binding activity, and transferrin receptor expression in the failing human heart</h3> <stencila-code-chunk itemscope="" itemtype="http://schema.stenci.la/CodeChunk" data-execution_count="1" data-programminglanguage="python"> <pre class="language-python" itemscope="" itemtype="http://schema.stenci.la/CodeBlock" slot="text"><code>from math import ceil import pandas as pd from orkg import ORKG from scipy.stats import ttest_ind orkg = ORKG(host='https://orkg.org/orkg') orkg.templates.materialize_templates(['R12002', 'R12006', 'R12008']) tp = orkg.templates df = pd.read_csv('data.csv') tt = ttest_ind(df['non-failing heart (NF)'], df['failing heart (F)'], equal_var=False, nan_policy='omit') pvalue = tt.pvalue pvalue_ceil = ceil(pvalue * 1000) / 1000.0 tp.students_ttest( 'Statistically significant hypothesis test with IRE binding dependent variable on failing and non-failing hearts (p<{})'.format(pvalue_ceil), 'http://purl.obolibrary.org/obo/GO_0030350', # the study design dependent variable tp.pvalue('the p-value of the statistical hypothesis test (p<{})'.format(pvalue_ceil), tp.scalar_value_specification(pvalue)), 'data.csv', # the input dataset ).serialize_to_file('article.contribution.1.json', as_jsonld=True)</code></pre> <figure slot="outputs"> <pre class="language-text" itemscope="" itemtype="http://schema.stenci.la/CodeBlock"><code>Installing dependencies: 100%|████████████████████| 3/3 [00:01<00:00, 1.78it/s] </code></pre> </figure> </stencila-code-chunk> <p itemscope="" itemtype="http://schema.stenci.la/Paragraph">Consistent with previous reports <span itemscope="" itemtype="http://schema.stenci.la/CiteGroup"><cite itemscope="" itemtype="http://schema.stenci.la/Cite"><a href="#bib1"><span>1</span><span>Maeder et al.</span><span>2011</span></a></cite><cite itemscope="" itemtype="http://schema.stenci.la/Cite"><a href="#bib2"><span>2</span><span>Leszek et al.</span><span>2011</span></a></cite></span> iron concentration was significantly lower in LV tissue samples from patients with advanced heart failure than in LV tissue samples from unused donor hearts. As shown by electrophoretic mobility shift assays, IRE binding activity was significantly (p<0.001) reduced in failing hearts (most pronounced in patients with ischemic cardiomyopathy) (<a href="#fig1" itemscope="" itemtype="http://schema.stenci.la/Link">Figure 1</a>). Protein expression levels of the transferrin receptor were significantly lower in failing hearts than in the controls.</p> <figure itemscope="" itemtype="http://schema.stenci.la/Figure" id="fig1" title="Figure 1"> <label data-itemprop="label">Figure 1</label> <stencila-code-chunk itemscope="" itemtype="http://schema.stenci.la/CodeChunk" data-tags="" data-execution_count="2" data-programminglanguage="python"> <pre class="language-python" itemscope="" itemtype="http://schema.stenci.la/CodeBlock" slot="text"><code>df.plot.box()</code></pre> <figure slot="outputs"> <pre><output><AxesSubplot:></output></pre><img src="index.html.media/0" alt="" itemscope="" itemtype="http://schema.org/ImageObject"> </figure> </stencila-code-chunk> <figcaption> <h4 itemscope="" itemtype="http://schema.stenci.la/Heading" id="ire-binding-activity-for-non-failing-nf-and-failing-f-hearts">IRE binding activity for non-failing (NF) and failing (F) hearts.</h4> </figcaption> </figure> <section 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